Abcam
Anti-Nestin antibody
ab6142
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We do work on gene and protein expression changes (epigenetic modulators) in neuroglia differentiation and behaviour. This product was used to check the expression of nestin in proliferative and differentiated neuroblastoma cells (N2A cells). Nestin is a good neuronal stem cell marker and can be used to check the neural stemness.
Western Blot
Neuroblastoma (N2A) cells
1:500, overnight 4c,
5%BSA in 1xTBST(0.1%TritonX100)
Antimouse-HRP, 1:10000, 2h at room temperature
No tertiary incubation
Chem-doc was used to detect the chemiluminiscence
The Nestin levels are high in the proliferative stage of neuronal cells compared to the differentiated cells.
None
No non specific bands
Dilution is less (1:500) which consumes more antibody and leads to high cost
Good neural stem cell marker to use.