Immunoprecipitation of GFP-fusion protein

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Company:

Novus Biologicals

Product Name:

GFP Antibody 0.1 mg

Catalog Number:

NB600-308

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This antibody was used in a study that investigated the role of Calpain-A protease in the Dorsal/Cactus (NFkB/IkB) activities during Drosophila embryogenesis.

Experimental Design and Results Summary

Applications

Immunoprecipitation

Sample

S2 Cells lysate

Primary Incubation

Polyclonal anti-GFP antibody (1:1000)

Blocking Agent

Polyclonal anti-GFP antibody (1:1000), Novus Biologicals

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Western Blot, using polyclonal anti-GFP antibody (1:1000), Novus Biologicals

Results Summary

We used a tagged construct (Cactus-GFP) expressed in S2 cells and Drosophila embryos. The protein Cactus-GFP was detected by GFP antibody as a 90 kDa band.

Additional Notes

None

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Summary

The Good

Dilution at 1:1000 and minimal background.

The Bad

None

The Bottom Line

The polyclonal anti-GFP antibody worked excellently in Immunoprecipitation and Western Blot applications with minimal background.

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