AbD Serotec (a Bio-Rad company)
Dynein Cytoplasmic 74kDa
0100-0076
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The main purpose of our work is to identify new functional roles of candidate genes in the cellular network. In order to understand their functional roles, we downregulate the candidate genes in different cell lines and verify it with Western Blot by using specific antibodies.
Western Blot
T84, MCF7 whole cell lysates
Dilution: 1/1000, time: 1 hour, room temperature
5% milk powder solution in 1xTBST
Anti mouse HRP (Dilution: 1/5000, time: 30 min, room temperature )
No tertiary incubation
ECL detection
By using this antibody the specific band for the dynein intermediate chains is detected without any problem.
The antibody detects all dynein intermediate chain subunits.
Specific, strong signal at low concentration of the primary antibody
Nothing bad
This antibody would be a great choice for Western Blot analysis, if you want to detect all dynein intermediate chain subunits.