Mouse spleen cells restimulated with Survivin66–74 in absence or presence of anti-MHC I antibodies

We analyzed T-cell responses to TAAs and re-stimulated spleen cells from c-di-GMP–treated and control mice with immunodominantSurvivin66–74 peptide (GWEPDDNPI) in the absence or presence of anti- MHC class I antibodies. Survivin is strongly expressed by 4T1 tumor cells. We found a significant increase in the number of IFNg-producing CD8 T cells to TAA Survivin compared with that of the saline group, which was almost completely reduced when blocked with anti-MHC class I antibodies.

Results published in Journal of Cancer Immunology Research: Chandra D, Quispe-Tintaya W, Jahangir A, Asafu-Adjei D, Ramos I, Sintim HO, Zhou J, Hayakawa Y, Karaolis DK, Gravekamp C. STING ligand c-di-GMP improves cancer vaccination against metastatic breast cancer. Cancer Immunol Res. 2014 Sep;2(9):901-10. doi: 10.1158/2326-6066.CIR-13-0123. Epub 2014 Jun 9. PubMed PMID: 24913717; PubMed Central PMCID: PMC4264585.