Santa Cruz Biotechnology
Toll antibody (d-300)
sc-33741
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This antibody was used in a study that investigated the role of Dpp signaling in the Toll pathway during Drosophila embryogenesis.
Immunofluorescence
Drosophila embryos fixed with 5% formaldehyde (Sigma) in PBS for 25 minutes at room temperature..
Toll antibody (d-300; Santa Cruz Biotechnology; sc-33741), incubated overnight at 4 °C (1:100 dilution)
5% normal goat serum in PBT.
Alexa Fluor 488 Goat Anti-Rabbit (Life techonologies - 1:500 dilution) for 2h at room temperature.
NA
Confocal microscope
This antibody worked excellently in immunofluorescence technique without background.
This antibody worked very well in Western blot for Drosophila embryos sample. In addition, samples from Rhodnius and Ticks work well, however they showed some unspecific bands.
This antibody is very specific for Drosophila melanogaster
None
An excellent antibody that can be used in arthropods.