Very Good Anti-Human CD4 antibody

Dept of biomedical Sciences
TTUHSC El Paso
Post doc

Overall

Quality of Results

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Company:

BD Bioscience

Product Name:

Pacific Blue™ Mouse Anti-Human CD4

Catalog Number:

558116

Browse Similar Products: CD4 Antibodies

Human PBMC reconstituted NOD-scid IL2rγcnull mice (NSG mice), were sacrificed and single cell suspensions from the spleen were used for flow cytometry. This antibody was used for detecting human CD4 expression on human CD45 and CD3 expressing cells. The result showed a clear CD3+/CD4+ and CD3+/CD4- population which confirms the specificity of this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes from humanized mice

Primary Incubation

30 min

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD FACS Canto II

Results Summary

The data showed a clear CD4+ and CD4- population, which confirm the specificity of this antibody.

Additional Notes

None

Related Categories

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Summary

The Good

Great specificity

The Bad

Titration is required

The Bottom Line

Very good CD4 Pacific Blue conjugated antibody

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