Great Polymerase for PCR

University of Southern California
Dermatology
Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bioline

Product Name:

MyFi™ DNA Polymerase

Catalog Number:

BIO-21117

We were trying to amplify a large insert without background that would not yield non specific bands. MyFi worked out great since it did exactly that!

Experimental Design and Results Summary

Application

Amplification of large insert

Starting Material

Plasmid A with insert into a lentivector

Protocol Overview

MyFi™ DNA Polymerase is great! In fact results were better than with Taq polymerases. We tried loading different initial DNA, temperatures, and times to anneal or time to denature. This polymerase worked well for every condition thrown at it.

Tips

None!

Results Summary

Clean non- specific amplification of large inserts

Additional Notes

None

Related Categories

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Summary

The Good

Lack of non-specific bands

The Bad

None

The Bottom Line

Totally worth my time and effort!

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