Resolving and identifying protein of interest using pageruler prestained protein ladder as a marker

indian institute of chemical technology
chemical biology

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Company:

Thermo Scientific

Product Name:

PageRuler Prestained Protein Ladder

Catalog Number:

26616

We checked neural protein expression using this protein ladder as a marker for proteins of various molecular weights. It is prestained and has multicoloured peptides which makes it easy to identify the band of the protein of interest.

Experimental Design and Results Summary

Application

SDS PAGE, Western blot

Starting Material

Cell lysate

Protocol Overview

To a 1.5 mm gel with 15 well combs (8% gel) add 4μl of protein ladder. Volume of protein ladder needs to be standardised as per your need for colour intensity of the protein ladder. Bands of the protein ladder resolve as per the % of SDS-PAGE gel

Tips

Store in -20 C, aliquot for regular use.

Results Summary

The proteins of 170-35kda are resolved in 8% gel, using the protein ladder for reference bands.

Additional Notes

None

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Summary

The Good

Prestained multicoloured ladder

The Bad

High cost

The Bottom Line

To find the different molecular weights of proteins in Western blots, this ladder helps for easy identification in the blot and in SDS-PAGE gel. Recommended for use.

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