eBioscience
Anti mouse Il-1b APC eFLour 780
47-7114-80
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We study cytokine production by macrophages in the presence of inflammation. We grow bone marrow derived macrophages and isolate primary microglia from mice and detect cytokine production by flow cytometry.
Flow Cytometry
Bone marrow macrophages
Manufacturer recommended, 15 minutes, room temperature
PBS, 1%BSA
NA
BD Canto II
We saw great separation of peaks between our FMO control and experimental groups, and were able to obtain relevant mean fluorescence intensity values for our experiment.
None
Great antibody!
Conjugated antibody - some care must be given for spectral overlap
Great for detection of IL-1b!