It extracts DNA but it is not pure

University of Miami
Cell Biology
Graduate Student

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Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

QIAquick Gel Extraction Kit

Catalog Number:

28704

I used this Gel DNA recovery kit to extract DNA from agarose fragments that were subsequently used for cloning purposes.

Experimental Design and Results Summary

Application

Gel DNA Recovery

Starting Material

None

Protocol Overview

Cut out agarose slab containing the DNA. Use kit to recover DNA. Check concentration and purity on Nanodrop.

Tips

Ethanol precipitate all the recovered DNA

Results Summary

The kit recovered the DNA but the 260/230 were extremely low, which indicates contamination.

Additional Notes

None

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Summary

The Good

Recovers DNA

The Bad

Contamination issues

The Bottom Line

This kit will recover the DNA but it will also carry over contaminants. This requires further purification by ethanol precipitation to remove them.

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