Sigma-Aldrich
Cobalt(II) chloride hexahydrate
255599
I used this CoCl2 to mimic hypoxia in primary epithelial cells without having to put them in a low oxygen environment to try and understand the role hypoxia plays in different cell functions.
Western Blot
HeLa cells
Make 100mM Cobalt chloride stock solution and then dilute 1:100 in cell culture media. Add media to cells and then incubate for 48 hrs and then measure HIF-1a protein levels from cell lysates
Be sure to dilute your stock solution because too much can be toxic
After adding cobalt chloride, HIF-1a protein was dramatically increased in Hela lysates compared to no cobalt chloride control.
None
Works well and increases HIF protein levels
This is a good source of cobalt chloride and I recommend it.