Use of Beckman Coulter centrifuge to centrifgue BV-2 cells into a pellet

May 05, 2015

University of Nebraska Medical Center
Pharmacology and Experimental Neuroscience
Graduate Research Assistant

Overall

Performance

Ease-of-Optimization

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Company:

Beckman Coulter

Product Name:

Allegra 6R centrifuge

Catalog Number:

366802

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I am conducting research on Parkinson’s disease, looking specifically at how effector T cells are able to modulate and exacerbate the neurodegeneration seen in the substantia nigra of Parkinson’s patients. The Beckman Coulter centrifuge is used multiple time during the passage of BV-2 cells in to a new flask in order to pellet the cells so that the media may be removed from the tube.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

BV-2 cells

Protocol Overview

BV-2 cells are released from the plate with the help of EDTA, media is then transfered to a 50 ml conical tube, and the tube is centrifuged at 500g for 5 minutes in order to pellet the cells to the bottom of the tube thereby allowing for the removal of the EDTA containing media from the cells.

Tips

Be sure to balance the machine correctly

Results Summary

The Beckman Coulter centrifuge performs consistently and creates a firm pellet of cells at the bottom of the tube.

Features Summary

None

Additional Notes

None

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Summary

The Good

This machine is very consistent

The Bad

If you mainly work in g, you must constantly convert to RPM when you use it.

The Bottom Line

The machine works wonderfully and is a good option if you use a centrifuge both at room temperature and at 4 degrees.

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