BioLegend
Alexa Fluor® 700 anti-mouse CD8a Antibody
100730
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The aim of my research is to study lymph node cells in cancer. In my experiment, I tested if a cytokine associated with tumor promotion alters cell counts. We selected CD8a Antibody from Biolegend specifically because CD8a is expressed in T cells and can be found in lymph nodes. Using this product I was able to differentiate T cell subsets.
Flow Cytometry
Lymph node
1:300 30' in ice
FcBlock
NA
Fluorescence
Need to be careful with APC, APC-Cy7, Bv711
None
Important reagent to have
Not very bright
Can be useful in multicolor panels