Very Good Anti-Human HLA-DR antibody

Dept of biomedical Sciences
TTUHSC El Paso
Post doc

Overall

Quality of Results

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Company:

BD Biosciences

Product Name:

HLA-DR antibody

Catalog Number:

560652

Browse Similar Products: HLA-DR Antibodies

Human PBMC reconstituted NOD-scid IL2rγcnull mice (NSG mice), were sacrificed and single cell suspensions from the spleen was used for flow cytometry. This antibody was used for detecting human HLA-DR expression on human CD45 and CD3 gated T cells isolated from these humanized mice. The result showed a clear HLA-DR+ population which confirms the specificity of this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes from humanized mice

Primary Incubation

30 min

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD FACS Canto II

Results Summary

Positive staining confirms the specificity of this antibody

Additional Notes

None

Related Categories

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Summary

The Good

Very good clear signal and reproducibility

The Bad

Titration is required

The Bottom Line

Good antibody for detecting human HLA-DR expression

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