Very Good Anti-Human CD8 antibody

Dept of biomedical Sciences
TTUHSC El Paso
Post doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

FITC anti-human CD8 Antibody

Catalog Number:

555366

Browse Similar Products: CD8 Antibodies

NOD-scid IL2rγcnull mice (NSG mice) reconstitute with human PBMCs were sacrificed and single cell suspensions prepared from spleen were used for flow cytometric analysis. The cells were stained with human CD45 and CD8 antibody. The staining looks good with two separate populations of CD8 positive and negative cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes from humanized mice

Primary Incubation

30 min

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry on FITC Channel

Results Summary

Clear separation of CD8 positive and negative cells which confirm the specificity of this antibody

Additional Notes

None

Related Categories

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Summary

The Good

Very good clear signal and reproducibility

The Bad

Titration is required

The Bottom Line

Good antibody for detecting human CD8 expression

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