Very good antibody for detecting human Cd4 expression

Dept of biomedical Sciences
TTUHSC El Paso
Post doc

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Quality of Results

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Company:

Tonbo Bioscience

Product Name:

PE anti-human CD4 Antibody

Catalog Number:

50-0048

Browse Similar Products: CD4 Antibodies

Single cell suspensions prepared from the spleen of human PBMC reconstituted mice was used for flow cytometric analysis. I have used this antibody to separate human CD4 positive T cells from the spleen of these humanized mice. The cells were stained with human CD45 and CD4 antibody and analyzed by flow cytometry. The staining looks pretty good with two separate populations of CD4+ and CD4- cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes from humanized mice

Primary Incubation

30 min

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD FACS Canto II

Results Summary

Clear Cd4 positive and negative population which confirm the specificity of this antibody

Additional Notes

None

Related Categories

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Summary

The Good

Very good clear signal and reproducibility

The Bad

None

The Bottom Line

Good antibody for detecting CD4 expression

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