Very Good Anti-Human CD45 antibody

Dept of biomedical Sciences
TTUHSC El Paso
Post doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Tonbo Bioscience

Product Name:

FITC anti-human CD45 Antibody

Catalog Number:

35-0459

Browse Similar Products: CD45 Antibodies

Single cell suspensions prepared from the spleen of human PBMC reconstituted mice was used for flow cytometric analysis. This antibody was used to separate human cells from the spleen of these humanized mice. The cells were stained with this CD45 antibody and analyzed by flow cytometry. The staining looks pretty good with two separate populations of CD45+ and CD45- cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes from humanized mice

Primary Incubation

30 min

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry on FITC Channel

Results Summary

Excellent staining for CD45 positive cells. This antibody can be used for detection and isolation of human CD45 positive cells.

Additional Notes

None

Related Categories

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Summary

The Good

Great specificity

The Bad

None

The Bottom Line

Very good antibody to detect CD45 expression

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