Bio-Rad
iTaq™ Universal SYBR® Green Supermix
172-5121
Info: Supplier Page
I was using this to perform quantitative PCR on a cDNA sample or a no template control. The cDNA sample showed amplification but the no template did not. I like that it is a 2X mix and have never had any trouble with the product i.e. the positive and negative controls regularly work.
PCR
cDNA sample or no template control
Mix 2X iTaq with primers and water to make a master mix. Load master mix into PCR plate and then add sample (I typically add 1-2ul of sample). Run PCR.
Be sure the iTaq is well mixed before making master mix
The data shown was taken on a CFX 96 and shows a good amplification curve and also shows a single melt peak.
None
Easy to use
I like this product and would recommend it.