ROR levels in human lung tissue

Environ. Med.
University of Rochester
Postdoc

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Company:

Abcam

Product Name:

Anti-ROR alpha antibody

Catalog Number:

ab60134

Info: View Product View Product Specs

Browse Similar Products: ROR-alpha Antibodies

We are evaluating the changes in clock proteins in wild type and clock deficient mutant mice to understand their role in specific pathological processes. We determined the cell-specific staining using IHC for ROR alpha antibody from Abcam.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Human lung sections

Primary Incubation

1:250 dilution in blocking buffer

Blocking Agent

10% Donkey serum

Secondary Incubation

1:1000 dilution Biotinylated Goat Anti-Rabbit IgG Antibody

Tertiary Incubation

DAB substrate

Detection

Light microscopy

Results Summary

We clearly observed staining of ROR alpha in different lung cells in human tissue stained for ROR alpha.

Additional Notes

None

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Summary

The Good

Works well for human samples

The Bad

None noted

The Bottom Line

Works well for IHC staining in human tissues

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