Tryple- Alternative to trypsin

University of Miami
Cell Biology
RA3

Overall

Quality of Results

Ease-of-Optimization

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Company:

Life Technologies

Product Name:

Tryple

Catalog Number:

12604-013

This product can be used for very fast dissociation of hard to trypsinize cells without the need to inactivate with FBS or SBTI. The tryple reagent is also very gentle on cells, it is stable at room temperature (no need to freeze like trypsin). In our case  it is great  for dissociating primary human bronchial epithelial cells.

Experimental Design and Results Summary

Application

Cell dissociation

Starting Material

Cell samples

Protocol Overview

Add tryple reagent and in 1-2 minutes cells start dissociating

Tips

None

Results Summary

Great product to dissociate cells without the need for a deactivation step

Additional Notes

None

Image Gallery

Summary

The Good

Very easy to do, fast dissociation

The Bad

None

The Bottom Line

Alternative for trypsin.

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