Abcam
Notch1 [EP1238Y] antibody
ab52627
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I was using this antibody to detect endogenous levels of Notch1 in primary epithelial cells from whole cell lysates. The antibody recognized a single band at the molecular weight shown from the company and had little background signal.
Western blot
Primary Human Epithelial Cells
1:1000, Overnight, 4 degrees
3% BSA
HRP at 1:5000 for 1 hr at room temperature
NA
Chemiluminescence
The Western blot showed a strong single band signal with low background. This indicates the primary antibody has good specificity in primary epithelial cells.
None
Detects Notch1 without need for optimisation
This is a good antibody to detect Notch1 and I would recommend it.