Rev-erb A alpha/NR1D1 Antibody for Immunohistochemistry

Environ. Med.
University of Rochester
Postdoc

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Quality of Results

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Company:

Novus Biologicals

Product Name:

Rev-erb A alpha/NR1D1 Antibody

Catalog Number:

H00009572-M02

Info: View Product View Product Specs

Browse Similar Products: NR1D1 Antibodies

We are evaluating the changes in clock proteins in wild type and clock deficient mutant mice to understand their role in specific pathological processes. Nr1d1 expression in mouse lungs was measured to determine cell-type specific staining of NR1D1 in vivo.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Paraffin embedded mouse lung sections

Primary Incubation

1:250

Blocking Agent

10% Donkey serum

Secondary Incubation

1 hour at room temp

Tertiary Incubation

NA

Detection

Fluorescence microscopy

Results Summary

Lung cells expressing NR1D1 in different areas.

Additional Notes

None

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Summary

The Good

Good for immunofluorescence staining in tissues

The Bad

Does not work well at lower concentrations (1:100; too much background)

The Bottom Line

Works for human and mouse lung immunofluorescence

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