Thermo Scientific
SuperSignal West Dura Extended Duration Substrate
34076
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This product was used to check the differential protein expression in proliferation and differentiation of neuronal cells involved in neuronal protection. This SuperSignal West Dura Extended Duration Substrate gives high signal intensity with less time consumption.
Western blot
Cell lysate
The equal proportion of luminol and peroxide buffer need to be mixed. Add equally on the blot and take the signal with chemdoc instrument.
Optimise the time exposure as per the protein expression intesity
The protein of interest expression is changed in differential cell treatment
None
High signal inensity with less time consumption
Need to mix both solutions in equal proportions
Product is the best among others to use it for developing the Western blots
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