E-cadherin / CDH1 Antibody Immunofluorescence

Cell Biology
University of Miami
RA3

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Scientific Pierce

Product Name:

E-cadherin / CDH1 Antibody (4A2C7)

Catalog Number:

33-4000

Info: View Product View Product Specs

Browse Similar Products: CDH1 Antibodies

Cadherin formation is one of the steps among many in the process of airway epithelial cell differentiation. In our laboratory we use this antibody along with others to follow this process.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

NHBE

Primary Incubation

1:1000 in 3 % BSA 4 degrees overnight

Blocking Agent

3% BSA

Secondary Incubation

1:2000 goat anti mouse AF647 (Life Tech)

Tertiary Incubation

NA

Detection

Confocal microscopy

Results Summary

Images show great junction staining

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Great staining and also works great for Western blot

The Bad

None

The Bottom Line

Highly recommend this product to use for both IF and WB

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