Use of EDTA in the passage of BV-2 cells

April 06, 2015

University of Nebraska Medical Center
Pharmacology and Experimental Neuroscience
Graduate Research Assistant

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Company:

Corning Life Sciences

Product Name:

EDTA

Catalog Number:

46-034-CI

I am conducting research on Parkinson’s disease, looking specifically at how effector T cells are able to modulate and exacerbate the neurodegeneration seen in the substantia nigra of Parkinson’s patients. 1mM EDTA is used to detach BV-2 cells from a T75 flask.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

BV-2 cells

Protocol Overview

1ml of 0.5M EDTA is added to 500ml of PBS to make 1mM concentration of EDTA. 3ml of this solution is then added to a T75 flask of BV-2 cells and incubated at 32 degrees celsius for 15 minutes. Plate is then gently tapped and cells release from the plate and are no longer attached.

Tips

Do not use more than 3ml of 1mM EDTA on the cells if passaging them at a 1:10 dilution

Results Summary

15 minutes of incubation with EDTA will cause at least 80-90% of the cells to detach from the T75 flask easily.

Additional Notes

None

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Summary

The Good

The EDTA will cause at least 80% of the cells to detach from the flask.

The Bad

If left on the cells for too long the EDTA will become very cytotoxic and kill the cells

The Bottom Line

EDTA is a great tool to use to detach cells from a flask in order to passage them.

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