Cell Signaling Technology
LSD1 (C69G12) Rabbit mAb
2184S
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Nuclear and cytoplasmic proteins from human dermal fibroblasts were separated using salt buffers and centrifugation methods. Separated nuclear and cytoplasmic protein (5ugs) was loaded onto a Western blot gel (4-12% gradient) and accurate separation was tested using anti-LSD1 antibody. Anti-LSD1 antibody was selected based on internet searches and publications.
Western Blot
Nucelar and cytoplasmic protein from human dermal fibroblasts
1:1000 in 5% skim milk in TBS-tween, 4 degrees, overnight
5% skim milk for 1 hour at room temperature
1:5000 anti-rabbit HRP-conjugated secondary (invitrogen) in 5% skim milk for 2h at room temperature
NA
Enhanced Chemiluminescence (Millipore) 2mins
Clear separation of nuclear (lanes 1, 3, and 5) and cytoplasmic fractions (lanes 2, 4 and 6) in human dermal fibroblasts (3 separate donor samples). One clear band is observed at 110kDa (as expected).
None
Clear single band
Great antibody to show nuclear separation