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PE/Cy5 anti-human CD62P (P-Selectin) Antibody
304908
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This antibody was used to determine if washed human platelets were activated after exposure to thrombin. After purification, platelets were incubated with or without 1UI/ml thrombin for 30mins at 37 degree C. Platelets were washed and stained with this anti-CD62P mAb for 45 minutes at 4 degree C followed by analysis on a flow cytometer. Platelets incubated with thrombin showed activation as measured by the increase in CD62P expression on the platelet surface. I would highly recommend this antibody for measuring human CD62P expression.
Flow Cytometry
Washed human platelets
1:100, 45 minutes at 4 degrees C
Buffer with BSA
NA
Thrombin activated platelets showed increase in CD62P expression as compared to non-activated platelets as shown in the figure.
None
Easy detection. Negligible background.
Great antibody!