Superb CD166 antibody!

Toxicology
University of Kentucky
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-human CD166 Antibody

Catalog Number:

343904

Info: View Product View Product Specs

Browse Similar Products: CD166 Antibodies

Detecting cancer stem cells is never easy. Scientists usually use a selection of antibodies. Our lab chose CD166 (which has been recently identified as a potential cancer stem cell marker). We used it on a pancreatic cancer cell line that was grown as spheres and expressed the general characteristics of stem cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PC3 pancreatic cancer cell line

Primary Incubation

No dilution, 20 minutes, on ice incubation

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry (FL-2 channel)

Results Summary

The antibody showed a distinctly strong signal in comparison to the PE-IgG isotype control.

Additional Notes

None

Related Categories

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Summary

The Good

Strong signal, no background, easy optimization

The Bad

None

The Bottom Line

For all scientists who are trying to identify cancer stem cells, this antibody will make a welcome addition to the repertoire of markers you use.

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