Santa Cruz Biotechnology
cytochrome c Antibody (A-8)
sc-13156
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We are investigating the role of cytochrome c during different apoptosis paradigms. One of the goals is to determine the subcellular localization of cytochrome c during apoptosis. To detect its intracellular localization, mouse embryonic fibroblasts were stained with cytochrome c antibodies.
Immunofluorescence
Mouse embryonic fibroblasts
1:50 dilution, room temperature incubation fo 3 hours
1x HBSS + 0.02% Tween-20 + 1.5% fetal bovine serum
Alexafluor 488 goat anti-rabbit IgG 1:1000 dilution for 3 hours at room temperature
N/A
Individual cells were visualized using a Nikon Eclipse Ti confocal microscope and a PlanApo 60x, 1.42 NA oil immersion objective.
The cytochrome c antibody staining generated high-quality intracellular distribution of mitochondria.
None
This is an excellent antibody for immunofluorescent staining.
Excellent antibody.