Agar-based plates

Northwestern University
Molecular Biosciences
Research associate

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Quality of Results

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Company:

US Biological

Product Name:

Agar

Catalog Number:

A0930-05

Our lab studies natural variation in stress response (to all different perturbations) in C. elegans. To carry out this research, we maintain hundreds of wild isolate C. elegans strains on agar-based plates. Any given experiment requires 500-1000 of these plates. We choose US Biological's agar to make the media for these plates because it is a great price and the company was great to work with. They sent us a free sample so we could test the agar in our recipe before we purchased the material in bulk.

Experimental Design and Results Summary

Application

Animal Maintenance

Starting Material

Wild isolate C. elegans strains 

Protocol Overview

We use the agar in preparing all the solid media plates in the lab. We have a simple recipe that involves agar, agarose, NaCl and peptone (which we also purchase from US Biological). The materials are autoclaved and then we added extra salts and cholesterol once the media is cooled. The combined solutions are then poured into 6 cm or 10 cm culture plates. These plates are used to maintain our C. elegans strains.

Tips

Product works great!

Results Summary

We have been using the agar since we started our lab two years ago. It has always worked well for our plate-pouring purposes.

Additional Notes

None

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Summary

The Good

Great price and good quality

The Bad

None

The Bottom Line

We intend to keep purchasing our agar from US Biological.

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