UltraComp beads- great results for mouse lymphocytes

Fred Hutchinson Cancer Research Center
Vaccine and Infectious Disease Division
Staff Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

UltraComp beads

Catalog Number:

01-2222-42

We use the UltraComp beads for single stained controls in three 13-color flow panels. It is important for our studies to be able compare changes in laser parameters over a period of time and standardize our sample collection for easier data analysis down the line.

Experimental Design and Results Summary

Application

Flow cytometry standardization

Starting Material

Mouse lymphocytes

Protocol Overview

We use the beads as single-stained controls for all antibodies in our three 13 color panels.

Tips

Dilute the antibodies to match the dilution used in staining cells for optimum results.

Results Summary

The beads work great to give us single stained controls to use for compensation and analysis that are standardized for better comparison between experiments and minimizing batch effects.

Additional Notes

None

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Summary

The Good

Quick, easy way to obtain SS controls

The Bad

Need to make sure dilution factor is correct

The Bottom Line

Beads work great and we have been using them for over 1.5 years!

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