Use of IL-6 to polarize naive CD4+ T cells

March 19, 2015

University of Nebraska Medical Center
Pharmacology and Experimental Neuroscience
Graduate Research Assistant

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Company:

eBioscience

Product Name:

Mouse Il-6 Recombinant Protein

Catalog Number:

14-8061-80

The cytokine IL-6 was used in culture with CD4+ T cells to polarize them to a Th17 phenotype.

Experimental Design and Results Summary

Application

cell culture

Starting Material

CD4+ naive T cells

Protocol Overview

Splenocytes were extracted from mice, and negatively selected for CD4+ T cells. The CD4+ naive T cells were then cultured with a milieu of cytokines and antibodies that included eBioscience's mouse IL-6 recombinant protein for 5 days.

Tips

Read the literature carefully to ensure that you are using the correct concentration of the cytokine IL-6 in your culture.

Results Summary

Almost 95% of the cells were polarized to the TH17 phenotype when analyzed by flow analysis after being in culture with IL-6 for 5 days.

Additional Notes

None

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Summary

The Good

This cytokine works well for TH17 polarization

The Bad

If the optimal concentration of IL-6 is not used in culture the cells will not proliferate nor polarize as well to the specific phenotype of interest.

The Bottom Line

The eBioscience cytokine IL-6 works well in the polarization of naive CD4+ T cells to the Th17 phenotype.

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