eBioscience
Anti-CD4 (clone 4SM95)
14-9766
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We are the histopathological service project in two Collaborative Research Centers and deal with various different mouse models of inflammation and infection Until now, the staining of mouse CD4 was only possible on cryo sections that are inferior to paraffin sections with respect to preservation of morphology and disinfection. With the anti-CD4 from eBioscience we are now able to stain CD4-positive cells in paraffin sections of mouse tissue. The best results are obtained after antigen retrieval using citrate buffer (pH 6). For detection, the LSAB method or immunofluorescence are suitable.
Immunohistochemistry
Paraffin sections of any mouse tissue
1:200, 30 minutes, room temperature
None
Biotinylated rabbit anti-rat (1:1000), 30 minutes, room temperature
Streptavidin-AP, 30 minutes, room temperature
ABC from Dako
The best results were obtained with an antigen retrieval using citrate buffer though EDTA buffer is also applicable. The staining after EDTA retrieval is less crisp and has more background.
The main advantage is that this anitbody works on paraffin sections
If you want to stain CD4 on mouse paraffin sections, this is the only antibody doing this.