US Biological
5-Fluoroorotic Acid Monohydrate (FOA, 5-FOA)
F5050
I have used this for fungal transformation; it worked pretty nicely. I would like to order this product again should I ever need to do that experiment.
Fungal transformation
Trichoderma reesei QM6a
Have to check the presence/absence of pyr4 gene in T. reesei genome. By using 5-FOA, we could able to check whether the dUMP->dTMP pathway is blocked or not. In another way, if T. reesei survives that indicates the presence of pyr4 gene and 5-FOA is not toxic to cells.
Fungal transformation check
It worked for me when doing homologous transformation by inserting and deleting of pyr4 genes.
None
Useful product
Not found yet
Worked well in my research; cheap compared to other companies