Reliable RIP3/RIPK3 Antibody

Immunobiology Laboratory
ULB
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Company:

Novus Biologicals

Product Name:

RIP3/RIPK3

Catalog Number:

NBP1-77299

Info: View Product View Product Specs

Browse Similar Products: RIP3/RIPK3 Antibodies

RIPK3 is a key protein involved in a novel form of regulated necrosis termed necroptosis. The inhibition of RIPK3 expression is an efficient strategy to suppress the pathway of necroptosis. This pAb to mouse RIP3/RIPK3 works very well in Western blotting at about 1/1000. It has now replaced the Imgenex #5523-2.

Experimental Design and Results Summary

Applications

Western Blot

Sample

L929 cells

Primary Incubation

1:1000

Blocking Agent

TBS Tween BSA 5%

Secondary Incubation

Anti-rabbit (Roche)

Tertiary Incubation

NA

Detection

ECL Pierce

Results Summary

As shown using shRNA to RIP3, the antibody is specific. There is no aspecific band when using the Ab between 1:500-1:1000.

Additional Notes

Saturation with TBS tween non-fat milk 5% also works.

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Summary

The Good

It is specific!!

The Bad

It is expensive

The Bottom Line

A reliable antibody which you need if you are interested in RIP3 biology (necroptosis etc.)

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