Using CD3/CD28 Beads for T cell Stimulation

March 10, 2015

University of Nebraska Medical Center
Pharmacology and Experimental Neuroscience
Graduate Student

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Quality of Results

Ease-of-Optimization

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Company:

Life Technologies

Product Name:

Dynabeads® Human T-Activator CD3/CD28

Catalog Number:

11131D

The aim of my research is to address the role of T cell subsets in both animal models of Parkinson's disease and human subjects. The experiments that use Dynabeads are for regulatory T cell functional assays to determine ability of the cells to inhibit or suppress effector subset proliferation. I chose Human T-activaor CD3/CD28 beads to stimulate my cultured T cells to keep them simulated, alive and proliferating.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

CD4+ T cells

Protocol Overview

The Dynabeads were used at a 1:8 ratio to stimulate CD4+ T cells isolated from patient blood for 3 days.

Tips

The beads are very easy to use. However, you must titrate them because it is easy to overstimulate your cells, resulting in too much proliferation or even death.

Results Summary

When using these Dynabeads for stimulation of isolated T cells via CD3 and CD28, I was able to successfully complete a regulatory T cell functional assay with reliable results. The beads did exactly what they were supposed to do!

Additional Notes

None

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Summary

The Good

Easy to use and lasts for a long time

The Bad

Titration is necessary, and it is possible to over-stimulate

The Bottom Line

I recommend the use of Dynabeads to stimulate T cells. I like using them much better than irradiated splenocytes for stimulation. I have also used the mouse beads, and they work just as well.

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