NeuN Antibody for Western Blot

chemical biology
indian institute of chemical technology

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Quality of Results

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Company:

EMD Millipore

Product Name:

Anti-NeuN

Catalog Number:

MAB377

This product was used to monitor expression levels of NeuN upon N2A cell differentiation to study the Differentiation of neuroblstoma (N2A cells) to mature neurons in serum deprivation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

N2A cells (Mouse Neuroblastoma)

Primary Incubation

1:3000 in 5%BSA in 1xTBST(0.1% Tritonx-100), four degrees, overnight

Blocking Agent

5%BSA in 1xTBST(0.1% Tritonx-100)

Secondary Incubation

Goat anti-mouse-IgG-HRP 1:10000, 2h, Room temperature

Tertiary Incubation

NA

Detection

Luminol/peroxide buffer, vilber lourmat chem doc

Results Summary

The expression levels are changing in N2A differentiation

Additional Notes

None

Related Categories

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Summary

The Good

Easy to find the band of interest 48/46 kda

The Bad

Non-specificity

The Bottom Line

Recommended for use

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