eBioscience
F4/80 Antigen
14-4801-82
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Our aim was to decipher drug accumulation in peritoneal macrophages. In order to target peritoneal macrophages from B lympocytes in the peritoneal exudate, we wanted to obtain a specific macrophage marker to differentiate from the B-lympocytes via flow cytometry.
Flow Cytometry
Peritoneal exudate
1:10 (antibody:cell volume), 2 ug/500,000 cells, 30 minutes, 4 degrees C in low serum media
Fc block
None
NA
405 450 laser in flow cytometry
The antibody worked well in terms of detection of peritoneal macrophages relative to B-lympocytes and made gating very easy.
Ease of use, highly specific
None so far
Great Product. Will Recommend.