R&D Systems
Human Galectin-1 Affinity Purified Polyclonal Ab
AF1152
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Browse Similar Products: Galectin-1 Immunofluorescence Antibodies
Galectin-1 (gal-1) is an important molecule secreted by many tumors which induces apoptosis in activated T-cells and promotes tumor angiogenesis, both phenomena that facilitate successful establishment of a tumor in the body. Here we test if we can detect gal-1 in human colon cancer cell line using confocal microscopy.
Immunofluorescence
Colon cancer cells
1:500 in block buffer
0.05% FBS in PBS
Alexa Fluor 488
NA
Using Zeiss confocal microscope
Gal-1 is detectable in the cytoplasm of the colon cancer cells as evidenced by the green color.
None
Very clean antibody
None observed
To detect gal-1 using IFC, this would be the best antibody.