BD
Precision Glide Neede 18G11/2
305196
After spleens have been removed from mice, a single cell suspension of splenocytes is performed using 18G Precision Glide Needles.
Cell work
Spleens
After spleens have been removed and smashed though a 70 micron filter, the 18G needle is attached to a 5ml syringe, next the splenocyte mixture is drawn up very slowly into the syringe and expelled very slowly into another 70 micron fliter on a 50 ml tube.
Draw up and expel the mixture very slowly to prevent lysis of your cells of interest
With the use of two 70 micron filters and the 18G needle attached to a 5ml syringe, the spleen cells were broken down from the spleen into a single cell suspension with very little extra material (such as fat tissue) making it into the final cell suspension.
NA
It works like a dream everytime
None that I have seen yet.
This product is very useful for producing a single cell suspension from tissue/organs removed from an animal.