Great antibody for detecting human CD4 expression

Dept of biomedical Sciences
TTUHSC El Paso
Post doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-human CD4 Antibody

Catalog Number:

317416

Human PBMC reconstituted NOD-scid IL2rγcnull mice (NSG mice) were sacrificed and single cell suspensions prepared from the spleens were used for flow cytometric analysis. This antibody was used for detecting human CD4 expression on human CD45 gated cells isolated from these humanized mice. The results showed a clear CD4 positive and negative population which confirms the specificity of this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes from humanized mice

Primary Incubation

30 minutes

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry on APC Channel

Results Summary

The results in the figure show a clear positive and negative population for expression of surface CD4.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Very good, clear signal and reproducibility.

The Bad

None

The Bottom Line

Can be used to detect Human Cd4 T cells.

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