Anti-Human CD87 (UPAR) APC, 100 tests from eBioscience

February 27, 2015

Biology
University of Ottawa
PhD student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Human CD87 (UPAR) APC 100 tests from eBioscience

Catalog Number:

17-3879-42

My project requires testing of CD87 positive and negative cells with aptamers. Positive cells co-express mCherry but to confirm a receptor (CD87) aptamers that bind to these antibodies were purchased. These were used to confirm CD87 expression which already was detected by aptamers and other antibodies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

200000 HEK 293 CELLS: CD87 positive/negative

Primary Incubation

24C, 30-60 min, 5 ul per sample (as indicated on the supplier website)

Blocking Agent

BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Beckman Gallios, FL6

Results Summary

No difference between positive and negative cells was observed.

Additional Notes

None

Related Categories

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Summary

The Good

None

The Bad

The supplier doesn't provide detailed instructions for this product.

The Bottom Line

No detailed recommendations on how to use these antibodies are provided. Last reference article is dated to 2003 which has no practical information on how to use this product.

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