Santa Cruz Biotechnology
PDI Antibody (H-160)
sc-20132
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We are investigating the role of PDI during ER stress-induced apoptosis. One of our goals is to determine the subcellular localization of PDI during apoptosis. To detect its intracellular localization, mouse embryonic fibroblasts were stained with PDI antibodies.
Immunofluorescence
Mouse embryonic fibroblasts
1:50 dilution, room temperature incubation fo 3 hours
1x HBSS + 0.02% Tween-20 + 1.5% fetal bovine serum
Alexafluor 594 goat anti-rabbit IgG 1:1000 dilution for 3 hours at room temperature
NA
Individual cells were visualized using a Nikon Eclipse Ti confocal microscope and a PlanApo 60x, 1.42 NA oil immersion objective.
The PDI antibody staining generated high-quality intracellular distribution of the endoplasmic reticulum.
None
The fluorescent singal is excellent.
This antibody is excellent for immunofluorescent staining.