Great control for immunoflorescence cell staining.

Pediatrics
UCSF
Junior Specialist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma Aldrich

Product Name:

Alpha-tubulin

Catalog Number:

T5168

Used in staining of wild type trophoblast stem cells. It was a good control to compare to our mutant stem cells. We picked this product because it gave us consistently good results in our previous staining experiments.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Wild type trophoblast stem cells

Primary Incubation

1:1000 at room temperature for 1 hour

Blocking Agent

5% BSA

Secondary Incubation

1:500 at room temperature for 1 hour

Tertiary Incubation

NA

Detection

Fluorescence microscope

Results Summary

Very clear staining, no background. Really specific for target.

Additional Notes

None

Related Categories

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Summary

The Good

Great and clear staining.

The Bad

Expensive!

The Bottom Line

Great antibody for staining tubulin.

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