Thermo Scientific
Pierce G2 fast blotter
88217
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This product is used to transfer proteins from sds-page gel to PVDF membranes of 45kda and ~14 kda proteins. Compared to wet transfer it reduces the time (15min 20v), consumes less transfer buffer (40ml/gel from Thermo), and is more user friendly.
Western blotting
SDS-PAGE gel, PVDF membrane, whatman paper, semidry buffer
Keep the gel in milliQ water for 10 minutes, activate the PVDF with methanol for 1 minute and place in semidry trasfer along with whatman paper. Arrange gel, PVDF and whatman paper and set the voltage and time. (20v-15min)
Standardize the voltage and time as per the protein molecular weight which needs to be transferred.
It transfers the protein from SDS-PAGE to PVDF completely, but sometimes the bands diffuse due to excess heat and more amount buffer.
The higher version of this which has display of running voltage and time
None
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Saves time, consumes less buffer, user friendly.
Standardize the temperature and time as per the protein to be transferred and no time and voltage display.
Time-saving for people who regularly do Western blot.
Product Name: G2 Fast BlotterSupplier Page