Cell Signaling Technology
Phospho-Vimentin (Ser83) Antibody
3878P
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Vimentin is a cytoskeletal protein that is increasingly gaining importance in the field of cancer research. It is important to know the post-translational status of vimentin that will provide us novel insights into its regulation at different compartments. Vimentin is known to be phosphorylated at various motifs, however there are very few antibodies available to detect them on Western blot. Here we test Ser83 clone.
Western Blot
Mouse cell lines
1:100 BSA
5% BSA
1:1000 HRP
NA
Western blotting
As detected the cell lines did show the presence of vimentin at 54 kDa and also a oligomer at 100kDa. However, the background is clean.
None
Very clean antibody
Higher forms are they background or oligomers?
Better antibody and very clean.