R&D Systems
Human Galectin-3 Affinity Purified Polyclonal Antibody
AF1154
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This product was used for detection of Galectin-3 in human colon epithelial cells. Galectin-3 is a lectin that is highly expressed in cancers and is also found in normal epithelial cells. To detect its expression in tissues, it is essential to find an antibody that does not over stain the background and is highly limited to the epithelial cells in the crypts. This antibody is very good for staining the epithelial cells.
Immunohistochemistry
Human colon tissue
1:500 in blocking buffer
Fish gelatin or 0.5% FBS in PBS
DAB staining using Vectastain
NA
IX81 microscope
Very good staining of the epithelial cells, however there is some background visible in the stromal component at lower dilutions.
None
Very clean antibody
Polyclonal
Very good detection for a polyclonal antibody