BD Pharmigen CD11c HL3 Clone

DBS
University of Montana
Graduate Student

Overall

Quality of Results

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Company:

BD Biosciences (Pharmigen)

Product Name:

PE Hamster Anti-Mouse CD11c

Catalog Number:

561044

Browse Similar Products: CD11c Flow Cytometry Antibodies

Our experiments assess the tendencies of environmental pollutants to affect dendritic cells' protein expression. We incubated Bone-Marrow Derived DCs with pollutant and collected data via flow cytometry. We used the HL3 clone because it appears to give cleaner results than the N418 clone.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

BMDCS

Primary Incubation

1.5 uL of .2mg/mL of antibody per million cells, incubate for 25 minutes at 4 celsius

Blocking Agent

FC Block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACSAria II

Results Summary

The PE mAB Anti-CD11c HL3clone gave clean results with very little non-specific binding. It maintained its fluorescence for at least 24 hours post labelling.

Additional Notes

None

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Summary

The Good

Clean results

The Bad

None

The Bottom Line

I would recommend this product, and the HL3 clone for assessing CD11c expression.

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