Life Technologies
Calretinin
18-0291
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Browse Similar Products: Calretinin Immunohistochemistry Antibodies
Mouse calretinin antibody from Invitrogen was tried on mouse ovary tissue successfully. IHC staining on mouse tissues for this antibody was not reported in the data sheet. Mouse on mouse staining makes this experiment a little difficult. But blocking with goat serum as well as incubating the primary antibody with 1% serum helped to get a good outcome.
Immunohistochemistry
Mouse ovary (control and Tumor)
1:200 dilution in 1% goat serum , overnight at 4 C
10% goat serum
Biotinylated Goat anti mouse RTU secondary from Invitrogen, room temperature for 60 minutes
AEC Chromogen
Streptavidin HRP and AEC chromogen
Six months control ovarian surface epithelium expressed calretinin, stained red in the cytoplasm while the experimental tumor sample shows lack of expression and negative staining in its surface epithelium.
Dysregulated estrogen receptor signaling in the hypothalamic-pituitary-ovarian axis leads to ovarian epithelial tumorigenesis in mice. Laws MJ, Kannan A, Pawar S, Haschek WM, Bagchi MK, Bagchi IC. PLoS Genet. 2014 Mar 6;10(3)
Good antibody for detection in mouse tissues.
Mouse on mouse staining is always tricky. Background staining is difficult to avoid.
Overall, it is a good antibody to work with in both mouse and human tissue sections.