Sigma Aldrich
Alpha-tubulin
T5168
Browse Similar Products: Alpha-tubulin Western blot Antibodies
Sigma's alpha-tubulin mouse monoclonal antibody is really great. While it is expensive, since this is a fairly ubiquitously expressed protein, you can easily get away with dilutions of 1:5000 and above for Western immunoblotting and immunocytochemistry (at least in our experimental setting). The results are very clear and do not seem to change between different lots. I would highly recommend this antibody for anyone looking for a reliable, strong loading control (for Westerns) as well as an indicator of tubulin structure (ICC/IHC).
Western Blot
Stem cells
1:5000 overnight at 4 C
Licor Odyssey PBS Blocking Buffer
1:15000 1 hour at room temperature with Licor Secondary Antibodies.
N/A
Licor Odyssey System
Very clear and clean Western image.
None
Very strong and can be used at 1:5000 and above, consistent between lots, great in ICC/IHC as well.
Expensive!
Great antibody that is worth the expense.